Análisis metagenómico del microbioma intestinal en lechones lactantes con consorcio bacteriano nativo y su impacto en peso y diarreas

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DOI:

https://doi.org/10.51252/revza.v5i1.1085

Palabras clave:

caracterización genómica, lechones destetados, PCR, probióticos

Resumen

El objetivo del estudio fue identificar la composición del microbioma intestinal en lechones lactantes tratados con un consorcio bacteriano nativo y evaluar su efecto en la ganancia de peso y la incidencia de diarreas. El experimento se realizó en 120 lechones de una granja porcina localizada en San Martín, distribuidos en tres grupos: Probiótico (recibió 1 ml de consorcio bacteriano nativo [3*10⁸ UFC/ml]), Excipiente (1 ml de agua) y Control (sin intervención). El periodo de estudio fue de 15 días. Para el análisis metagenómico, se seleccionaron cinco lechones por grupo, tomando muestras del duodeno mediante hisopado. La identificación del microbioma se realizó mediante PCR. En los lechones del grupo Probiótico se identificaron géneros bacterianos como Lactobacillus, Actinobacillus, Alloprevotella, Clostridium sensu stricto 1, entre otros. Los resultados mostraron que este grupo obtuvo mayor ganancia de peso (1.99 kg) y no presentó casos de diarrea, a diferencia del grupo Excipiente. Se concluye que la administración del consorcio bacteriano nativo fue beneficiosa al mejorar significativamente la ganancia de peso e inhibir la aparición de diarreas en lechones lactantes durante su etapa crítica de desarrollo.

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Xin J, Zeng D, Wang H, Sun N, Zhao Y, Dan Y, et al. Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance, Intestinal Immunity, and Gut Microbiota in Piglets. Probiotics Antimicrob Proteins [Internet]. 2020 Mar 7;12(1):184–93. Available from: http://link.springer.com/10.1007/s12602-018-9511-y

Wang K, Zhu Q, Kong X, Song M, Azad MAK, Xiong L, et al. Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets. Front Vet Sci [Internet]. 2020 Dec 14;7. Available from: https://www.frontiersin.org/articles/10.3389/fvets.2020.597832/full

McCormack UM, Curião T, Buzoianu SG, Prieto ML, Ryan T, Varley P, et al. Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs. Dudley EG, editor. Appl Environ Microbiol [Internet]. 2017 Aug;83(15). Available from: https://journals.asm.org/doi/10.1128/AEM.00380-17

He Y, Kim K, Kovanda L, Jinno C, Song M, Chase J, et al. Bacillus subtilis: a potential growth promoter in weaned pigs in comparison to carbadox. J Anim Sci [Internet]. 2020 Sep 1;98(9). Available from: https://academic.oup.com/jas/article/doi/10.1093/jas/skaa290/5900678

Sarkar VK, De UK, Kala A, Verma AK, Chauhan A, Paul BR, et al. Early-Life Intervention of Lactoferrin and Probiotic in Suckling Piglets: Effects on Immunoglobulins, Intestinal Integrity, and Neonatal Mortality. Probiotics Antimicrob Proteins [Internet]. 2023 Feb 6;15(1):149–59. Available from: https://link.springer.com/10.1007/s12602-022-09964-y

Yue S, Li Z, Hu F, Picimbon JF. Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding. Sci Rep [Internet]. 2020 Nov 10;10(1):19476. Available from: https://www.nature.com/articles/s41598-020-75207-1

Crespo-Piazuelo D, Estellé J, Revilla M, Criado-Mesas L, Ramayo-Caldas Y, Óvilo C, et al. Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions. Sci Rep [Internet]. 2018 Aug 24;8(1):12727. Available from: https://www.nature.com/articles/s41598-018-30932-6

Ramayo-Caldas Y, Mach N, Lepage P, Levenez F, Denis C, Lemonnier G, et al. Phylogenetic network analysis applied to pig gut microbiota identifies an ecosystem structure linked with growth traits. ISME J [Internet]. 2016 Dec 1;10(12):2973–7. Available from: https://academic.oup.com/ismej/article/10/12/2973-2977/7538136

Dudík B, Kiňová Sepová H, Bilka F, Pašková Ľ, Bilková A. Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells. Antonie Van Leeuwenhoek [Internet]. 2020 Aug 14;113(8):1191–200. Available from: https://link.springer.com/10.1007/s10482-020-01426-1

Gardiner GE, Metzler-Zebeli BU, Lawlor PG. Impact of Intestinal Microbiota on Growth and Feed Efficiency in Pigs: A Review. Microorganisms [Internet]. 2020 Nov 28;8(12):1886. Available from: https://www.mdpi.com/2076-2607/8/12/1886

Lin CF, Lin MY, Lin CN, Chiou MT, Chen JW, Yang KC, et al. Potential probiotic of Lactobacillus strains isolated from the intestinal tracts of pigs and feces of dogs with antibacterial activity against multidrug-resistant pathogenic bacteria. Arch Microbiol [Internet]. 2020 Sep 23;202(7):1849–60. Available from: https://link.springer.com/10.1007/s00203-020-01908-w

Gilbert H, Billon Y, Brossard L, Faure J, Gatellier P, Gondret F, et al. Review: divergent selection for residual feed intake in the growing pig. Animal [Internet]. 2017;11(9):1427–39. Available from: https://linkinghub.elsevier.com/retrieve/pii/S175173111600286X

Zhang S, Zhang H, Zhang C, Wang G, Shi C, Li Z, et al. Composition and evolutionary characterization of the gut microbiota in pigs. Int Microbiol [Internet]. 2023 Nov 20;27(4):993–1008. Available from: https://link.springer.com/10.1007/s10123-023-00449-8

Xu T, Sun H, Yi L, Yang M, Zhu J, Huang Y, et al. Comparing the taxonomic and functional profiles of gut microbiota from three pig breeds by metagenomic sequencing. Front Genet [Internet]. 2022 Oct 14;13. Available from: https://www.frontiersin.org/articles/10.3389/fgene.2022.999535/full

Herrmann E, Young W, Reichert-Grimm V, Weis S, Riedel C, Rosendale D, et al. In Vivo Assessment of Resistant Starch Degradation by the Caecal Microbiota of Mice Using RNA-Based Stable Isotope Probing—A Proof-of-Principle Study. Nutrients [Internet]. 2018 Feb 6;10(2):179. Available from: https://www.mdpi.com/2072-6643/10/2/179

Looft T, Allen HK, Cantarel BL, Levine UY, Bayles DO, Alt DP, et al. Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations. ISME J [Internet]. 2014 Aug 1;8(8):1566–76. Available from: https://academic.oup.com/ismej/article/8/8/1566-1576/7582538

Stojanov S, Berlec A, Štrukelj B. The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease. Microorganisms [Internet]. 2020 Nov 1;8(11):1715. Available from: https://www.mdpi.com/2076-2607/8/11/1715

Xu M, Tian P, Zhu H, Zou R, Zhao J, Zhang H, et al. Lactobacillus paracasei CCFM1229 and Lactobacillus rhamnosus CCFM1228 Alleviated Depression- and Anxiety-Related Symptoms of Chronic Stress-Induced Depression in Mice by Regulating Xanthine Oxidase Activity in the Brain. Nutrients [Internet]. 2022 Mar 18;14(6):1294. Available from: https://www.mdpi.com/2072-6643/14/6/1294

Shen J, Zhang J, Zhao Y, Lin Z, Ji L, Ma X. Tibetan Pig-Derived Probiotic Lactobacillus amylovorus SLZX20-1 Improved Intestinal Function via Producing Enzymes and Regulating Intestinal Microflora. Front Nutr [Internet]. 2022 Mar 29;9. Available from: https://www.frontiersin.org/articles/10.3389/fnut.2022.846991/full

Reyer H, Sjöberg PJR, Oster M, Wubuli A, Murani E, Ponsuksili S, et al. Mineral Phosphorus Supply in Piglets Impacts the Microbial Composition and Phytate Utilization in the Large Intestine. Microorganisms [Internet]. 2021 Jun 1;9(6):1197. Available from: https://www.mdpi.com/2076-2607/9/6/1197

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Publicado

2025-01-20

Cómo citar

Rodríguez-Rufasto, S. D., Puicón-Niño-de-Guzmán, V. H., & Lopez-Flores, A. M. (2025). Análisis metagenómico del microbioma intestinal en lechones lactantes con consorcio bacteriano nativo y su impacto en peso y diarreas. Revista De Veterinaria Y Zootecnia Amazónica, 5(1), e1085. https://doi.org/10.51252/revza.v5i1.1085

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