Metagenomic analysis of the gut microbiome in suckling piglets with native bacterial consortium and its impact on weight and diarrhea
DOI:
https://doi.org/10.51252/revza.v5i1.1085Keywords:
genomic characterization, weaned piglets, PCR, probioticsAbstract
The objective of this study was to identify the composition of the gut microbiome in suckling piglets treated with a native bacterial consortium and evaluate its effect on weight gain and diarrhea incidence. The experiment was conducted on 120 piglets from a pig farm located in San Martín, distributed into three groups: Probiotic (received 1 ml of native bacterial consortium [3*10⁸ CFU/ml]), Excipient (1 ml of water), and Control (no intervention). The study period was 15 days. For metagenomic analysis, five piglets per group were selected, and duodenal samples were swabbed. Microbiome identification was performed by PCR. Bacterial genera such as Lactobacillus, Actinobacillus, Alloprevotella, and Clostridium sensu stricto 1, among others, were identified in the piglets in the Probiotic group. The results showed that this group achieved greater weight gain (1.99 kg) and did not present any cases of diarrhea, unlike the Excipient group. It is concluded that the administration of the native bacterial consortium was beneficial by significantly improving weight gain and inhibiting the occurrence of diarrhea in suckling piglets during their critical stage of development.
Downloads
References
Xin J, Zeng D, Wang H, Sun N, Zhao Y, Dan Y, et al. Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance, Intestinal Immunity, and Gut Microbiota in Piglets. Probiotics Antimicrob Proteins [Internet]. 2020 Mar 7;12(1):184–93. Available from: http://link.springer.com/10.1007/s12602-018-9511-y
Wang K, Zhu Q, Kong X, Song M, Azad MAK, Xiong L, et al. Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets. Front Vet Sci [Internet]. 2020 Dec 14;7. Available from: https://www.frontiersin.org/articles/10.3389/fvets.2020.597832/full
McCormack UM, Curião T, Buzoianu SG, Prieto ML, Ryan T, Varley P, et al. Exploring a Possible Link between the Intestinal Microbiota and Feed Efficiency in Pigs. Dudley EG, editor. Appl Environ Microbiol [Internet]. 2017 Aug;83(15). Available from: https://journals.asm.org/doi/10.1128/AEM.00380-17
He Y, Kim K, Kovanda L, Jinno C, Song M, Chase J, et al. Bacillus subtilis: a potential growth promoter in weaned pigs in comparison to carbadox. J Anim Sci [Internet]. 2020 Sep 1;98(9). Available from: https://academic.oup.com/jas/article/doi/10.1093/jas/skaa290/5900678
Sarkar VK, De UK, Kala A, Verma AK, Chauhan A, Paul BR, et al. Early-Life Intervention of Lactoferrin and Probiotic in Suckling Piglets: Effects on Immunoglobulins, Intestinal Integrity, and Neonatal Mortality. Probiotics Antimicrob Proteins [Internet]. 2023 Feb 6;15(1):149–59. Available from: https://link.springer.com/10.1007/s12602-022-09964-y
Yue S, Li Z, Hu F, Picimbon JF. Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding. Sci Rep [Internet]. 2020 Nov 10;10(1):19476. Available from: https://www.nature.com/articles/s41598-020-75207-1
Crespo-Piazuelo D, Estellé J, Revilla M, Criado-Mesas L, Ramayo-Caldas Y, Óvilo C, et al. Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions. Sci Rep [Internet]. 2018 Aug 24;8(1):12727. Available from: https://www.nature.com/articles/s41598-018-30932-6
Ramayo-Caldas Y, Mach N, Lepage P, Levenez F, Denis C, Lemonnier G, et al. Phylogenetic network analysis applied to pig gut microbiota identifies an ecosystem structure linked with growth traits. ISME J [Internet]. 2016 Dec 1;10(12):2973–7. Available from: https://academic.oup.com/ismej/article/10/12/2973-2977/7538136
Dudík B, Kiňová Sepová H, Bilka F, Pašková Ľ, Bilková A. Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells. Antonie Van Leeuwenhoek [Internet]. 2020 Aug 14;113(8):1191–200. Available from: https://link.springer.com/10.1007/s10482-020-01426-1
Gardiner GE, Metzler-Zebeli BU, Lawlor PG. Impact of Intestinal Microbiota on Growth and Feed Efficiency in Pigs: A Review. Microorganisms [Internet]. 2020 Nov 28;8(12):1886. Available from: https://www.mdpi.com/2076-2607/8/12/1886
Lin CF, Lin MY, Lin CN, Chiou MT, Chen JW, Yang KC, et al. Potential probiotic of Lactobacillus strains isolated from the intestinal tracts of pigs and feces of dogs with antibacterial activity against multidrug-resistant pathogenic bacteria. Arch Microbiol [Internet]. 2020 Sep 23;202(7):1849–60. Available from: https://link.springer.com/10.1007/s00203-020-01908-w
Gilbert H, Billon Y, Brossard L, Faure J, Gatellier P, Gondret F, et al. Review: divergent selection for residual feed intake in the growing pig. Animal [Internet]. 2017;11(9):1427–39. Available from: https://linkinghub.elsevier.com/retrieve/pii/S175173111600286X
Zhang S, Zhang H, Zhang C, Wang G, Shi C, Li Z, et al. Composition and evolutionary characterization of the gut microbiota in pigs. Int Microbiol [Internet]. 2023 Nov 20;27(4):993–1008. Available from: https://link.springer.com/10.1007/s10123-023-00449-8
Xu T, Sun H, Yi L, Yang M, Zhu J, Huang Y, et al. Comparing the taxonomic and functional profiles of gut microbiota from three pig breeds by metagenomic sequencing. Front Genet [Internet]. 2022 Oct 14;13. Available from: https://www.frontiersin.org/articles/10.3389/fgene.2022.999535/full
Herrmann E, Young W, Reichert-Grimm V, Weis S, Riedel C, Rosendale D, et al. In Vivo Assessment of Resistant Starch Degradation by the Caecal Microbiota of Mice Using RNA-Based Stable Isotope Probing—A Proof-of-Principle Study. Nutrients [Internet]. 2018 Feb 6;10(2):179. Available from: https://www.mdpi.com/2072-6643/10/2/179
Looft T, Allen HK, Cantarel BL, Levine UY, Bayles DO, Alt DP, et al. Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations. ISME J [Internet]. 2014 Aug 1;8(8):1566–76. Available from: https://academic.oup.com/ismej/article/8/8/1566-1576/7582538
Stojanov S, Berlec A, Štrukelj B. The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease. Microorganisms [Internet]. 2020 Nov 1;8(11):1715. Available from: https://www.mdpi.com/2076-2607/8/11/1715
Xu M, Tian P, Zhu H, Zou R, Zhao J, Zhang H, et al. Lactobacillus paracasei CCFM1229 and Lactobacillus rhamnosus CCFM1228 Alleviated Depression- and Anxiety-Related Symptoms of Chronic Stress-Induced Depression in Mice by Regulating Xanthine Oxidase Activity in the Brain. Nutrients [Internet]. 2022 Mar 18;14(6):1294. Available from: https://www.mdpi.com/2072-6643/14/6/1294
Shen J, Zhang J, Zhao Y, Lin Z, Ji L, Ma X. Tibetan Pig-Derived Probiotic Lactobacillus amylovorus SLZX20-1 Improved Intestinal Function via Producing Enzymes and Regulating Intestinal Microflora. Front Nutr [Internet]. 2022 Mar 29;9. Available from: https://www.frontiersin.org/articles/10.3389/fnut.2022.846991/full
Reyer H, Sjöberg PJR, Oster M, Wubuli A, Murani E, Ponsuksili S, et al. Mineral Phosphorus Supply in Piglets Impacts the Microbial Composition and Phytate Utilization in the Large Intestine. Microorganisms [Internet]. 2021 Jun 1;9(6):1197. Available from: https://www.mdpi.com/2076-2607/9/6/1197
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Sandra Dioscórida Rodríguez-Rufasto, Víctor Humberto Puicón-Niño-de-Guzmán, Alicia Maria Lopez-Flores
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors retain their rights:
a. The authors retain their trademark and patent rights, as well as any process or procedure described in the article.
b. The authors retain the right to share, copy, distribute, execute and publicly communicate the article published in the Revista de Veterinaria y Zootecnia Amazónica (REVZA) (for example, place it in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the REVZA.
c. Authors retain the right to make a subsequent publication of their work, to use the article or any part of it (for example: a compilation of their works, notes for conferences, thesis, or for a book), provided that they indicate the source of publication (authors of the work, journal, volume, number and date).
