Extracción de pectina a partir de cáscara de cacao de diferentes genotipos y estados de maduración
DOI:
https://doi.org/10.51252/raa.v5i2.939Palabras clave:
Caracterización de biopolímeros, Compuestos de alto metoxilo, Variabilidad genotípica, Tecnologías de extracción verde, Bioeconomía circularResumen
Este estudio evalúa la extracción y caracterización fisicoquímica de pectina obtenida de cáscara de vaina de cacao (CPH) de dos genotipos (CCN51 e IMC-67) en diferentes estados de madurez. Se optimizaron múltiples variables de extracción para maximizar el rendimiento y la calidad del gel, incluida la concentración de agua acidulada, el pH, la temperatura y el tiempo de exposición. Los resultados mostraron que el genotipo y la madurez afectan significativamente la eficiencia de extracción y la funcionalidad de la pectina. El mayor rendimiento de pectina fue del 77,7% para el IMC-67 (maduro) y del 72,6% para el CCN51 (semimaduro) en condiciones óptimas (pH 1,5-1,6, 80 °C, 75 minutos). El análisis fisicoquímico reveló un alto contenido de ácido galacturónico (hasta 82,3%), un grado de esterificación superior al 50% y una excelente consistencia en gel, clasificando la pectina como de alto metoxilo. Estos hallazgos respaldan la valorización sostenible de los subproductos agroindustriales del cacao para posibles aplicaciones alimentarias, farmacéuticas y de envasado.
Descargas
Citas
Adi-Dako, O., Ofori-Kwakye, K., Frimpong Manso, S., Boakye-Gyasi, M. EL, Sasu, C., & Pobee, M. (2016). Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical. Journal of Pharmaceutics, 2016, 1-12. https://doi.org/10.1155/2016/7608693 DOI: https://doi.org/10.1155/2016/7608693
Alcantara, G. A. P., Gaban, P. J. V., Rivadeneira, J. P., & Castillo-Israel, K. A. T. (2022). Citric Acid Method Optimization for Pectin Extraction from Unripe ‘Saba’ Banana (Musa acuminata X Musa balbisiana BBB) Peels. Materials Science Forum, 1069, 219-230. https://doi.org/10.4028/p-5zjw8x DOI: https://doi.org/10.4028/p-5zjw8x
Anoraga, S. B., Shamsudin, R., Hamzah, M. H., Sharif, S., Saputro, A. D., & Basri, M. S. M. (2024). Optimization of subcritical water extraction for pectin extraction from cocoa pod husks using the response surface methodology. Food Chemistry, 459, 140355. https://doi.org/10.1016/j.foodchem.2024.140355 DOI: https://doi.org/10.1016/j.foodchem.2024.140355
Ardiansyah, D., Komala, I., & Zilhadia, Z. (2021). Extraction of Pectin from Breadnut Fruit (Artocarpus altilis Fosberg.) with Citric Acid. Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia. https://doi.org/10.4108/eai.26-10-2020.2311324 DOI: https://doi.org/10.4108/eai.26-10-2020.2311324
Arioui, F., Ait Saada, D., & Cheriguene, A. (2017). Physicochemical and sensory quality of yogurt incorporated with pectin from peel of Citrus sinensis. Food Science & Nutrition, 5(2), 358-364. https://doi.org/10.1002/fsn3.400 DOI: https://doi.org/10.1002/fsn3.400
Arlorio, M., Coisson, J. D., Restani, P., & Martelli, A. (2001). Characterization of Pectins and Some Secondary Compounds from Theobroma cacao Hulls. Journal of Food Science, 66(5), 653-656. https://doi.org/10.1111/j.1365-2621.2001.tb04616.x DOI: https://doi.org/10.1111/j.1365-2621.2001.tb04616.x
Athawale Gauri, H., Raichurkar, S. J., & Dagadkhair, R. A. (2022). Pectate as a Gelling Agent in Foods (1.a ed.). CRC Press. DOI: https://doi.org/10.1201/9781003207689-10
Baltazar Flores, R., Carbajal Mariños, D., Baca Rodríguez, N., & Salvador Rodríguez, D. (2013). Optimization of the conditions of pectin extraction from lemon rind french (Citrus medica) using response surface methodology. Agroindustrial science, 77-89. https://doi.org/10.17268/agroind.science.2013.02.01 DOI: https://doi.org/10.17268/agroind.science.2013.02.01
Barazarte, H., Sangronis, E., & Unai, E. (2008). La cáscara de cacao (Theobroma cacao L.): Una posible fuente comercial de pectinas. Archivos Latinoamericanos de Nutrición, 58(1), 64-70. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0004-06222008000100009&lng=es&tlng=es
Barrios‐Rodríguez, Y. F., Salas‐Calderón, K. T., Orozco‐Blanco, D. A., Gentile, P., & Girón‐Hernández, J. (2022). Cocoa Pod Husk: A High‐Pectin Source with Applications in the Food and Biomedical Fields. ChemBioEng Reviews, 9(5), 462-474. https://doi.org/10.1002/cben.202100061 DOI: https://doi.org/10.1002/cben.202100061
Belkheiri, A., Forouhar, A., Ursu, A. V., Dubessay, P., Pierre, G., Delattre, C., Djelveh, G., Abdelkafi, S., Hamdami, N., & Michaud, P. (2021). Extraction, Characterization, and Applications of Pectins from Plant By-Products. Applied Sciences, 11(14), 6596. https://doi.org/10.3390/app11146596 DOI: https://doi.org/10.3390/app11146596
Blakemore, W. R., Dewar, E. T., & Hodge, R. A. (1966). Polysaccharides of the cocoa pod husk. Journal of the Science of Food and Agriculture, 17(12), 558-560. https://doi.org/10.1002/jsfa.2740171208 DOI: https://doi.org/10.1002/jsfa.2740171208
Campos-Vega, R., Nieto-Figueroa, K. H., & Oomah, B. D. (2018). Cocoa (Theobroma cacao L.) pod husk: Renewable source of bioactive compounds. Trends in Food Science & Technology, 81, 172-184. https://doi.org/10.1016/j.tifs.2018.09.022 DOI: https://doi.org/10.1016/j.tifs.2018.09.022
Chuenkaek, T., Vo, T. M. T., Nakajima, K., & Kobayashi, T. (2024). Pectin Materials Sourced From Agriculture Waste (pp. 285-320). https://doi.org/10.4018/979-8-3693-0003-9.ch011 DOI: https://doi.org/10.4018/979-8-3693-0003-9.ch011
Dewan, A., Malik, M., Shobhit, Chaudhary, V., & Sharma, A. (2024). Packaging Applications of Cocoa Pod Husk. En Agro-Wastes for Packaging Applications (pp. 264-287). CRC Press. https://doi.org/10.1201/9781003453277-12 DOI: https://doi.org/10.1201/9781003453277-12
FAOSTAT. (2025). Food and agriculture data. Food and Agriculture Organization of the United Nations. https://www.fao.org/faostat/en/#home
FCC. (2012). Pectin Monograph. Food Chemicals Codex. https://www.foodchemicalscodex.org/
García, L. C., Zambrano, E., Maddela, N. R., García, M. S., Aguilar, C., & Gavilánez, F. Z. (2024). Cacao Agribusiness in a Global Context. En Sustainable Cacao Cultivation in Latin America (pp. 3-15). Routledge. https://doi.org/10.4324/9781003381761-2 DOI: https://doi.org/10.4324/9781003381761-2
Hennessey-Ramos, L., Murillo-Arango, W., Vasco-Correa, J., & Paz Astudillo, I. C. (2021). Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L. Molecules, 26(5), 1473. https://doi.org/10.3390/molecules26051473 DOI: https://doi.org/10.3390/molecules26051473
ICCO. (2025). Statistics. The International Cocoa Organization. https://www.icco.org/statistics/
Khamsucharit, P., Laohaphatanalert, K., Gavinlertvatana, P., Sriroth, K., & Sangseethong, K. (2018). Characterization of pectin extracted from banana peels of different varieties. Food Science and Biotechnology, 27(3), 623-629. https://doi.org/10.1007/s10068-017-0302-0 DOI: https://doi.org/10.1007/s10068-017-0302-0
Le, Y., Cheng, L., Yang, Q., Shi, K., Han, F., Luo, W., & Duan, S. (2024). Source, Extraction, Properties, and Multifunctional Applications of Pectin: A Short Review. Polymers, 16(20), 2883. https://doi.org/10.3390/polym16202883 DOI: https://doi.org/10.3390/polym16202883
Levigne, S., Ralet, M.-C., & Thibault, J.-F. (2002). Characterisation of pectins extracted from fresh sugar beet under differeant conditions using an experimental design. Carbohydrate Polymers, 49(2), 145-153. https://doi.org/10.1016/S0144-8617(01)00314-9 DOI: https://doi.org/10.1016/S0144-8617(01)00314-9
Li, R., Fan, H., Li, B., Ge, J., Zhang, Y., Xu, X., Pan, S., & Liu, F. (2024). Comparison on emulsifying and emulgelling properties of low methoxyl pectin with varied degree of methoxylation from different de-esterification methods. International Journal of Biological Macromolecules, 263, 130432. https://doi.org/10.1016/j.ijbiomac.2024.130432 DOI: https://doi.org/10.1016/j.ijbiomac.2024.130432
Liew, S. Q., Chin, N. L., Yusof, Y. A., & Sowndhararajan, K. (2016). Comparison of Acidic and Enzymatic Pectin Extraction from Passion Fruit Peels and Its Gel Properties. Journal of Food Process Engineering, 39(5), 501-511. https://doi.org/10.1111/jfpe.12243 DOI: https://doi.org/10.1111/jfpe.12243
Mada, T., Duraisamy, R., & Guesh, F. (2022). Optimization and characterization of pectin extracted from banana and papaya mixed peels using response surface methodology. Food Science & Nutrition, 10(4), 1222-1238. https://doi.org/10.1002/fsn3.2754 DOI: https://doi.org/10.1002/fsn3.2754
Mamiru, D., & Gonfa, G. (2023). Extraction and characterization of pectin from watermelon rind using acetic acid. Heliyon, 9(2), e13525. https://doi.org/10.1016/j.heliyon.2023.e13525 DOI: https://doi.org/10.1016/j.heliyon.2023.e13525
Marsiglia-Lopez, D., Ramirez-Uribe, M., Gonzalez-Delgado, A., Ojeda-Delgado, K., & Sanchez-Tuiran, E. (2017). Computer-aided economic evaluation of pectin extraction from cocoa pod husk (Theobroma cacao L.). Contemporary Engineering Sciences, 10(30), 1493-1500. https://doi.org/10.12988/ces.2017.710140 DOI: https://doi.org/10.12988/ces.2017.710140
Maulani, T. R., Lioe, H. N., Arief, I. I., & Kusumaningrum, H. D. (2024). Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit. Jurnal Kimia Valensi, 10(1), 11-19. https://doi.org/10.15408/jkv.v10i1.37702 DOI: https://doi.org/10.15408/jkv.v10i1.37702
Meza-Sepulveda, D. C., Hernandez-Urrea, C., & Quintero-Saavedra, J. I. (2024). Physicochemical characterization of the pod husk of Theobroma cacao L. of clones CCN51, FEAR5, and FSV41 and its agroindustrial application. Heliyon, 10(7), e28761. https://doi.org/10.1016/j.heliyon.2024.e28761 DOI: https://doi.org/10.1016/j.heliyon.2024.e28761
Mollea, C., & Chiampo, F. (2019). Valorization of Cocoa Husks: Pectin Recovery. International Journal of Food Science, 2019, 1-5. https://doi.org/10.1155/2019/1212081 DOI: https://doi.org/10.1155/2019/1212081
Mounya, K. S., & Chowdary, A. R. (2024). Optimization of ultrasound‐assisted pectin recovery from cocoa by‐products using response surface methodology. Journal of the Science of Food and Agriculture, 104(11), 6714-6723. https://doi.org/10.1002/jsfa.13498 DOI: https://doi.org/10.1002/jsfa.13498
Musita, N.-. (2021). Characteristics of Pectin Extracted from Cocoa Pod Husks. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 37(1). https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v37i1.428 DOI: https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v37i1.428
Nopiani, Y., Hamzah, F. H., Pato, U., & Zamzani, dan M. (2024). The Effect of Extraction Temperature on the Physicochemical Characteristics of Pectin From Banana Bunch. IOP Conference Series: Earth and Environmental Science, 1364(1), 012073. https://doi.org/10.1088/1755-1315/1364/1/012073 DOI: https://doi.org/10.1088/1755-1315/1364/1/012073
Owusu, F. W. A., Boakye-Gyasi, M. E., Entsie, P., Bayor, M. T., & Ofori-Kwakye, K. (2021). Utilization of Pectin from Okra as Binding Agent in Immediate Release Tablets. BioMed Research International, 2021(1). https://doi.org/10.1155/2021/6002286 DOI: https://doi.org/10.1155/2021/6002286
Pasandide, B., Khodaiyan, F., Mousavi, Z., & Hosseini, S. S. (2018). Pectin extraction from citron peel: optimization by Box–Behnken response surface design. Food Science and Biotechnology, 27(4), 997-1005. https://doi.org/10.1007/s10068-018-0365-6 DOI: https://doi.org/10.1007/s10068-018-0365-6
Patel, P. M. (2017). Share Your Innovations through JACS Directory Journal of Natural Products and Resources Extraction of Pectin from Mango Peel and Application of Pectin. J. Nat. Prod. Resour, 3(1), 102-103. http://www.jacsdirectory.com/jnpr
Pinkaew, T., Inthachat, W., Khemthong, C., Kemsawasd, V., On-Nom, N., & Temviriyanukul, P. (2024). High Pectin Recovery from Cocoa Husks Using an Autoclave Approach: An Analysis of Its Physicochemical, Structural, and Genotoxicity Properties. Foods, 13(5), 669. https://doi.org/10.3390/foods13050669 DOI: https://doi.org/10.3390/foods13050669
Pranayasa, W. A., Putra, G. P. G., & Suhendra, L. (2022). Pengaruh Daya Gelombang Mikro Dan Rasio Bahan-Pelarut Pada Rendemen Dan Sifat Fisikokimia Pektin Dari Kulit Buah Kakao. Jurnal Rekayasa Dan Manajemen Agroindustri, 10(2), 184. https://doi.org/10.24843/JRMA.2022.v10.i02.p06 DOI: https://doi.org/10.24843/JRMA.2022.v10.i02.p06
Quiceno Suarez, A., Cadena-Chamorro, E. M., Ciro-Velásquez, H. J., & Arango-Tobón, J. C. (2024). By-products of the cocoa agribusiness: high valueadded materials based on their bromatological and chemical characterization. Revista Facultad Nacional de Agronomía Medellín, 77(1), 10585-10599. https://doi.org/10.15446/rfnam.v77n1.107738 DOI: https://doi.org/10.15446/rfnam.v77n1.107738
Ramli, N. (2011). Effect of ammonium oxalate and acetic acid at several extraction time and pH on some physicochemical properties of pectin from cocoa husks (Theobroma cacao). African Journal of Food Science, 5(5). https://doi.org/10.5897/AJFS11.107 DOI: https://doi.org/10.5897/AJFS11.107
Ranganna, S. (1986). Handbook of Analysis and Quality Control for Fruit and Vegetable Products. Tata McGraw-Hill.
Santos, E. S. B. d., Leal, I. L., & Nery, T. B. R. (2021). Extraction of Pectin from Epicarp and Mesocarp Fractions of Cocoa Shell. Journal of bioengineering and technology applied to health, 4(2), 52-57. https://doi.org/10.34178/jbth.v4i2.160 DOI: https://doi.org/10.34178/jbth.v4i2.160
Sarah, M., Hasibuan, I. M., Misran, E., & Maulina, S. (2022). Optimization of Microwave-Assisted Pectin Extraction from Cocoa Pod Husk. Molecules, 27(19), 6544. https://doi.org/10.3390/molecules27196544 DOI: https://doi.org/10.3390/molecules27196544
Shaibu, C. O., Dinshiya, J., & Shaibu, V. B. (2022). Short Communication: Extraction and characterization of pectin from ripe and unripe mango (Mangifera indica) peel. Asian Journal of Natural Product Biochemistry, 20(1). https://doi.org/10.13057/biofar/f200104 DOI: https://doi.org/10.13057/biofar/f200104
Tanaid, R. A. (2018). Screening of Variables Affecting Extraction of Pectin from Unripe Banana (Musa acuminata × balbisiana var. Cardaba) Peel using Plackett–Burman Design. Science and Humanities Journal, 12(1), 18-26. https://doi.org/10.47773/shj.1998.111.2 DOI: https://doi.org/10.47773/shj.1998.111.2
Vriesmann, L. C., Teófilo, R. F., & Lúcia de Oliveira Petkowicz, C. (2012). Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.) with citric acid. LWT, 49(1), 108-116. https://doi.org/10.1016/j.lwt.2012.04.018 DOI: https://doi.org/10.1016/j.lwt.2012.04.018
Wulandari, R., Suyatma, N. E., Budi, F. S., & Utami, R. R. (2023). Optimization of pectin extraction from cacao pods using microwave assisted extraction (MAE) as a sustainable method for on-farm waste utilization. IOP Conference Series: Earth and Environmental Science, 1241(1), 012082. https://doi.org/10.1088/1755-1315/1241/1/012082 DOI: https://doi.org/10.1088/1755-1315/1241/1/012082
Yapo, B. M., & Koffi, K. L. (2013). Extraction and Characterization of Gelling and Emulsifying Pectin Fractions from Cacao Pod Husk. Journal of Food and Nutrition Research, 1(4), 46-51. https://doi.org/10.12691/jfnr-1-4-3
Yusof, F., Khanahmadi, S., Amid, A., & Mahmod, S. S. (2016). Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross-linked enzyme aggregate. SpringerPlus, 5(1), 57. https://doi.org/10.1186/s40064-015-1621-3 DOI: https://doi.org/10.1186/s40064-015-1621-3

Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2025 Enrique Arévalo-Gardini, Cesar Oswaldo Arévalo-Hernández, Virupax Baligar, Dapeng Zhang

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Los autores retienen sus derechos:
a. Los autores retienen sus derechos de marca y patente, y tambien sobre cualquier proceso o procedimiento descrito en el artículo.
b. Los autores retienen el derecho de compartir, copiar, distribuir, ejecutar y comunicar públicamente el articulo publicado en la Revista Agrotecnológica Amazónica (RAA) (por ejemplo, colocarlo en un repositorio institucional o publicarlo en un libro), con un reconocimiento de su publicación inicial en la RAA.
c. Los autores retienen el derecho a hacer una posterior publicación de su trabajo, de utilizar el artículo o cualquier parte de aquel (por ejemplo: una compilación de sus trabajos, notas para conferencias, tesis, o para un libro), siempre que indiquen la fuente de publicación (autores del trabajo, revista, volumen, número y fecha).