Determination of DL50 of Ethyl Methanesulfonate (EMS) for the induction of morphological and physiological changes in Plukenetia volubuilis seedlings
DOI:
https://doi.org/10.51252/raa.v2i1.209Keywords:
phenotypic alterations, lethal dose, ethyl methanesulphonate, mutationsAbstract
The use of chemical mutagens is a widely used tool for the generation of new genetic variants in various agricultural crops. The use of Ethyl Methanesulphonate (EMS) in seeds of Plukenetia volubilis L. was evaluated to determine the optimal concentration of EMS that would reduce the germination and / or emergence of the seeds up to 50.0%, and to evaluate the morphological and physiological alterations in seedlings of P volubilis during the first generation. A simple DCA was used with different doses (0.0%, 0.5%, 1.0%, 1.5%, 2.0% and 3.0%) of EMS in a single exposure time (30 hrs.), plus an absolute control (seeds without treatment), to evaluate the mutagenic sensitivity of P. volubilis L., considering parameters such as percentage of emergence, plant height, loss of apical dominance, chlorosis and deformation of the leaves. The results showed that the 3.0% dose of EMS with 30 hours of exposure, reduced the emergence of seedlings by up to 50.0%, a value considered as the mean lethal dose (DL50) for P. volubilis. Likewise, phenotypic alterations such as leaf deformation, chlorosis, decrease in height and loss of apical dominance were evidenced with increasing EMS doses. These results demonstrate the potential of EMS to be used in sacha inchi seeds with the aim of generating new genetic variants of this species.
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