Inverse relationship between the number of blood draws and the osmotic fragility of sheep erythrocytes
DOI:
https://doi.org/10.51252/revza.v5i2.1190Keywords:
blood storage, animal welfare, sheep bloodAbstract
Sheep blood is an important biological reagent for clinical and microbiological laboratories. It is essential to maintain the cellular integrity of its erythrocytes when used in hemolytic reactions of diagnostic protocols. The objective of this study was to determine the relationship between the number of blood draws in rams and erythrocyte osmotic fragility, and its correlation with blood storage time at (2-8) °C, with its thermal robustness for 16 h at laboratory temperature (22-25) °C, and with the welfare and behavior of the rams. The experiments revealed that erythrocyte osmotic fragility is inversely related to the number of blood draws. Hemoglobin concentration remained within the normal range for up to six blood draws, and indicators of animal welfare and behavior did not change. Erythrocytes maintained their osmotic integrity for 42 days of storage at (2-8) °C and for up to 16 h at (22-25) °C. This work has demonstrated, for the first time, the resistance of sheep blood to hemolysis after repeated blood draws from the same animal or exposure to suboptimal temperatures, confirming its use as a reliable reagent in routine diagnostic procedures.
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