Control of varroasis in honey bees using a natural acaricide based on Neem oil, an ecological and effective solution
DOI:
https://doi.org/10.51252/revza.v4i2.750Keywords:
acaricide, apis mellifera, VarroaAbstract
The study lasted 5 weeks, during which different treatments with varying levels of neem oil were evaluated to control (Varroa destructor) in bee colonies. Four treatments with commercial neem oil were used at concentrations of 0%, 0.33%, 0.66%, and 1.32%, each applied to 4 bee colonies, totaling 16 experimental units. A Completely Randomized Design (CRD) was employed for the treatments and repetitions, with fumigations carried out every 7 days over the 5-week period, collecting data for each variable. At the end of the experiment, it was observed that the treatment with 1.32% neem oil (T3) recorded the lowest infestation rate in adult bees, reaching 13.7%. This treatment proved to be the most effective in reducing the infestation rate of Varroa in the colonies, confirming the effectiveness of neem oil as a natural and ecological acaricide in controlling Varroa destructor in honey bees.
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Papa G, Maier R, Durazzo A, Lucarini M, Karabagias IK, Plutino M, et al. The Honey Bee Apis mellifera: An Insect at the Interface between Human and Ecosystem Health. Biology (Basel). 2022 Feb 1;11(2):233. Disponible en: https://doi.org/10.3390/biology11020233
Grindrod I, Martin SJ. Parallel evolution of Varroa resistance in honey bees: a common mechanism across continents? Proc R Soc B Biol Sci. 2021 Aug 11;288(1956):20211375. Disponible en: https://doi.org/10.1098/rspb.2021.1375
Oddie MAY, Burke A, Dahle B, Le Conte Y, Mondet F, Locke B. Reproductive success of the parasitic mite (Varroa destructor) is lower in honeybee colonies that target infested cells with recapping. Sci Rep. 2021 Apr 28;11(1):9133. Disponible en: https://doi.org/10.1038/s41598-021-88592-y
Martin SJ, Hawkins GP, Brettell LE, Reece N, Correia-Oliveira ME, Allsopp MH. Varroa destructor reproduction and cell re-capping in mite-resistant Apis mellifera populations. Apidologie. 2020 Jun 10;51(3):369–81. Disponible en: https://doi.org/10.1007/s13592-019-00721-9
Perez AA, Johnson BR. Task repertoires of hygienic workers reveal a link between specialized necrophoric behaviors in honey bees. Behav Ecol Sociobiol. 2019 Sep 9;73(9):123. Disponible en: https://doi.org/10.1007/s00265-019-2731-7
Qadir ZA, Idrees A, Mahmood R, Sarwar G, Bakar MA, Ahmad S, et al. Effectiveness of Different Soft Acaricides against Honey Bee Ectoparasitic Mite Varroa destructor (Acari: Varroidae). Insects. 2021 Nov 17;12(11):1032. Disponible en: https://doi.org/10.3390/insects12111032
Melathopoulos AP, Winston ML, Whittington R, Smith T, Lindberg C, Mukai A, et al. Comparative Laboratory Toxicity of Neem Pesticides to Honey Bees (Hymenoptera: Apidae), Their Mite Parasites Varroa jacobsoni (Acari: Varroidae) and Acarapis woodi (Acari: Tarsonemidae), and Brood Pathogens Paenibacillus larvae and Ascophaera apis. J Econ Entomol. 2000 Apr 1;93(2):199–209. Disponible en: https://doi.org/10.1603/0022-0493-93.2.199
Gracia MJ, Moreno C, Ferrer M, Sanz A, Peribáñez MÁ, Estrada R. Field efficacy of acaricides against Varroa destructor. Blenau W, editor. PLoS One. 2017 Feb 3;12(2):e0171633. Disponible en: https://doi.org/10.1371/journal.pone.0171633
González-Gómez R, Otero-Colina G, Villanueva-Jiménez JA, Peña-Valdivia CB, Santizo-Rincón JA. Repellency of the oily extract of neem seeds (Azadirachta indica) against Varroa destructor (Acari: Varroidae). Exp Appl Acarol. 2012 Mar 24;56(3):261–70. Disponible en: https://doi.org/10.1007/s10493-012-9517-1
Jack CJ, Ellis JD. Integrated Pest Management Control of Varroa destructor (Acari: Varroidae), the Most Damaging Pest of ( Apis mellifera L. (Hymenoptera: Apidae)) Colonies. Li-Byarlay H, editor. J Insect Sci. 2021 Sep 1;21(5). Disponible en: https://doi.org/10.1093/jisesa/ieab058
Vera-Párraga JK. Control de varroasis en abejas melíferas mediante el uso de un acaricida natural con base en aceite de neem. Universidad Técnica Estatal de Quevedo; 2023.
Cuesta-Albertos JA, Nieto-Reyes A. The random Tukey depth. Comput Stat Data Anal. 2008 Jul;52(11):4979–88. Disponible en: https://doi.org/10.1016/j.csda.2008.04.021
Cifuentes LJC, Lira LAN, Hernández YCU, Luy-Montejo CA, Apaza YCL, Flores JEA, et al. Influence of SAS Software on the Learning of Statistics at the University Level. J Internet Serv Inf Secur. 2023 Aug 31;13(3):178–90. Disponible en: https://doi.org/10.58346/JISIS.2023.I3.011
Yépez-Rosado ÁJ, Morales-Cabezas DC, Yépez-Yánez ÁB, Urdanigo-Zambrano JP, Jimez-Romero EM, Chamorro-Changoquiza DC. Inclusión de harina de follaje de frejol de palo (Cajanus cajan L, Millsp) en dietas para pollos broilers y su impacto en la producción. Rev Vet y Zootec Amaz. 2024 May 30;4(1):e683. Disponible en: https://doi.org/10.51252/revza.v4i1.683
Airahuacho Bautista F, Jiménez Torres V, Rubina Airahuacho S, Velásquez Vergara C. Evaluación de productos alternativos naturales en el control de la Varroa destructor en abejas melíferas (Apis mellifera). Rev Investig Vet del Perú. 2023 Jun 29;34(3):e23741. Disponible en: https://doi.org/10.15381/rivep.v34i3.23741
Reyna Fuentes JH, Martínez González JC, Silva Contreras A, López Aguirre D. Efecto de tres moliendas vegetales contra el ácaro Varroa destructor en colonias de Apis mellifera. Nov Sci. 2022 May 26;14(28). Disponible en: https://doi.org/10.21640/ns.v14i28.3019
Díaz-Monroy B, Moyón-Moyón J, Baquero-Tapia MF. Evaluación de tres alternativas para el control de varroasis (Varroa destructor) en apiarios ecuatorianos. Cienc y Agric. 2019 Jan 6;16(1):63–78. Disponible en: https://doi.org/10.19053/01228420.v16.n1.2019.8832
Polo Corro JL, Alvarado Ibañez JC, Valderrama Alfaro SM. Determinación del índice de infestación por Varroa destructor en colonias de Apis mellifera, en condiciones naturales. Ambient Comport y Soc. 2022 Aug 11;5(1):55–68. Disponible en: https://doi.org/10.51343/racs.v5i1.799
Kolics É, Specziár A, Taller J, Mátyás KK, Kolics B. Lithium chloride outperformed oxalic acid sublimation in a preliminary experiment for Varroa mite control in pre-wintering honey bee colonies. Acta Vet Hung. 2021 Apr 19;68(4):370–3. Disponible en: https://doi.org/10.1556/004.2020.00060
Guerrero Catota RS. Evaluación de diferentes niveles de Cloruro de Litio en la alimentación artificial para el control varroa destructor en abejas (apis mellifera). Escuela Superior Politécnica de Chimborazo; 2019. Disponible en: http://dspace.espoch.edu.ec/handle/123456789/13315
Maya O, Medina C, Aquino G, Olmos G, López M. Tratamiento estacional con amitraz contra Varroa destructor y sus efectos en colonias de Apis mellifera. Abanico Vet. 2021;10(2):2448–6132. Disponible en: https://doi.org/10.21929/abavet2020.38
Moyón-Moyón J, Díaz-Monroy B, Baquero-Tapia MF. Evaluación de tres alternativas para el control de varroasis (Varroa destructor) en apiarios ecuatorianos. Cienc y Agric. 2019 Jan 6;16(1):63–78. Disponible en: https://doi.org/10.19053/01228420.v16.n1.2019.8832
Reyes F, Vargas J, Martos A, Chura J. Efectividad de cuatro acaricidas en el control de ácaro (Varroa destructor) en abejas (Apis mellifera L.). Universidad Nacional Agraria La Molina; 2020; 81(1): 229-242.
Villegas Martínez XA. Prevalencia y manejo integrado del ácaro Varroa destructor en colmenas de abejas africanizadas [Internet]. Universidad Nacional, Centro de Investigaciones Apícolas Tropicales (CINAT); 2019. Disponible en: https://catalogosiidca.csuca.org/Record/CR.UNA01000319269
Calderón RA, Ramírez M, Ramírez F, Villalobos E. Efectividad del ácido fórmico y el timol en el control del ácaro Varroa destructor en colmenas de abejas africanizadas. Agron Costarric. 1969;38(1). Disponible en: https://doi.org/10.15517/rac.v38i1.15165
Pomagualli Chafla CJ. Acaricidas sintéticos y naturales para el control de varroa destructor en colmenas apis mellifera [Internet]. Escuela Superior Politécnica de Chimborazo; 2017. Disponible en: http://dspace.espoch.edu.ec/handle/123456789/8140
Soledispa Yépez JN. Alternativas de control del ácaro Varroa spp en los panales de abejas en la provincia de Santa Elena [Internet]. Universidad Estatal Península de Santa Elena; 2018. Disponible en: https://repositorio.upse.edu.ec/handle/46000/4280
Ibarra Y. Niveles de ácido oxálico para el control de varroasis (Varroa destructor) EN ABEJAS (Apis mellifera), en el recinto Aguas Frias del cantón Mocache, año 2018”. Agric Food Sci Environ Sci [Internet]. 2019. Disponible en: https://www.semanticscholar.org/paper/Niveles-de-ácido-oxálico-para-el-control-de-(Varroa-Navarrete-Santiago/85dd6de42c24e7e0c9c118e8aa34ebdcfbb3baf8
Cruz I, Rodríguez G, Pérez MI, Ruíz J, de los Santos RB, Merino CG. Eficacia de timol para control de Varroa destructor en Apis Mellifera en Putla Villa De Guerrero, Oaxaca. Rev Mex Agroecosistemas [Internet]. 2023;X(S1):1–17. Disponible en: https://revistaremaeitvo.mx/index.php/remae/article/view/63
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Copyright (c) 2024 Ángel Joel Yépez-Rosado, Carmen Lizbeth Verdezoto-Michuy, Diana Carolina Morales-Cabezas, Ronald Roberto Cabezas-Congo, Angel Bolívar Yépez-Yánez, Jennifer Kasandra Vera-Párraga, Patricia Palma-Roxana, Jeison Daniel Zambrano-Flores, Angel Antonio Chamorro-Palacios
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