Molecular characterization and determination of the probiotic character of lactic acid bacteria isolated from the gastrointestinal microbiome of the growing chicken
DOI:
https://doi.org/10.51252/revza.v2i2.395Keywords:
16S rRNA, lactic acid bacteria, microbiota, chickenAbstract
The gastrointestinal microbiota of the chicken is a fundamental component of poultry production in physiological, zootechnical and veterinary terms. Worldwide, studies related to the chicken microbiota are presented as a main priority of the poultry sector with research focused on probiotic and / or pathogenic microorganisms. In the frame-work of the present work, the isolation was achieved in Man Rogose and Sharpe medium of 32 strains of lactic acid bacteria from the gastrointestinal microbiota. The partial sequencing of the 16S rRNA al-lowed the molecular identification of these three lactic acid bacterial strains from the ventricle (Weissella sp.), from the cecum (Pediococcus pentosaceos and Enterococcus faecium). To determine their probiotic nature, these bacterial strains were evaluated based on acid resistance tests (pH 2.5, 3.5 and 5), bile (0, 0.10, 0.15 and 0.30%) and in vitro antagonism against Salmonella typhimurium. The results contribute to the availability of new probiotic strains for the poultry production sector and to the modernization of the technologies for characterization and molecular identification of the microorganisms of the chicken microbiota.
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Copyright (c) 2022 Fredy Fabian-Dominguez, Miluska Vanessa Baylon-Cuba, Rosel Apaestegui-Livaque, Lourdes Vásquez-Rojas, Eric Mialhe
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