Effect of recombinant FSH on superovulation in high-producing Holstein cows in Huacho
DOI:
https://doi.org/10.51252/revza.v6i1.1448Keywords:
corpora lutea, heat stress, ovarian ultrasonography, reproductive hormonesAbstract
In recent years, the development of assisted reproductive biotechnologies in cattle, such as embryo transfer, increased its importance due to their potential to accelerate genetic improvement. However, their effectiveness depended largely on achieving an adequate superovulation, traditionally induced with pituitary-derived FSH, which presented limitations such as the risk of LH contamination, short half-life, and possible disease transmission. Therefore, the use of recombinant FSH (rFSH) emerged as a safer and potentially more effective alternative. The objective of the present study was to evaluate the effect of a recombinant follicle-stimulating hormone on superovulation in high-producing Holstein cows. The research was conducted between January and March 2023 at Establo Granados (Huacho, Lima), using an applied experimental design with two decreasing-dose hormonal protocols: 140 µg and 180 µg of rFSH. Eleven cows were selected under strict zootechnical and reproductive criteria. The results showed that the low dose (140 µg) generated a better ovarian response, with a higher number (4.8 CLs) and larger size of corpora lutea (18.58 mm), compared with the high dose (180 µg), which resulted in 2.83 corpora lutea with an average size of 13.87 mm. It was concluded that the low rFSH dose was more effective, although further validation with a larger number of animals was recommended.
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